王宏伟 博士

教授、博士生导师

1992-1996     威斯尼斯人老品牌官网生物科学与技术系，学士

1996-2001     威斯尼斯人老品牌官网生物科学与技术系，博士

2001-2006     美国劳伦斯伯克利国家实验室生命科学部，博士后

2006-2008     美国劳伦斯伯克利国家实验室生命科学部，研究科学家

2009-2011     美国耶鲁大学分子生物物理与生物化学系，Tenure-Track助理教授

2010.12-至今 威斯尼斯人老品牌官网，教授、博导

2016.04-2021.04   威斯尼斯人老品牌官网院长

●  研究兴趣、领域：

利用冷冻电子显微学研究生物大分子复合体的结构与分子机理。主要研究方向如下：

1. 更高效、更高分辨率的冷冻电镜方法学开发；

2. 核酸质量控制的分子机制和调控；

3. 细胞骨架和生物膜系统的协调机制。

●  代表性论文：

1. Zheng LM, Liu N*, Gao XY, Zhu WQ, Liu K, Wu C, Yan R, Zhang JC, Gao X, Yao YT, Deng B, Xu J, Lu Y, Liu, ZM, Li MS, Wei XD*, Wang HW*, Peng HL*. (2023) Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nat Methods, 20(1):123-130.

2. Lu Y, Liu N*, Liu Y, Zheng LM, Yang JH, Wang J, Jia X, Zi QR, Peng HL*, Rao Y*, Wang HW*. (2022) Functionalized graphene grids with various charges for single-particle cryo-EM. Nat. Commun., 13(1):6718.

3. Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li XM, Huang Y, Wang HW*, Ma JB*. (2022) Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. Nature, 607(7918):399-406.

4. Zheng L, Liu N, Liu Y, Li N, Zhang J, Wang C, Zhu W, Chen Y, Ying D, Xu J, Yang Z, Gao X, Tang J, Wang X, Liang Z, Zou R, Li Y, Gao P, Wei X*, Wang HW*, Peng HL*. (2021) Atomically Thin Bilayer Janus Membranes for Cryo-electron Microscopy. ACS Nano, 15(10):16562-16571.

5. Liu N, Zheng LM, Xu J, Wang J, Hu CX, Lan J, Zhang X, Zhang J, Xu K, Cheng H, Yang Z, Gao X, Wang X, Peng HL*, Chen YN*, Wang HW*. (2021) Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM. Biophysics Reports, 7(3): 227-238. (Cover)

6. Jin W, Wang J, Liu CP, Wang HW*, Xu RM*. (2020) Structural Basis for pri-miRNA Recognition by Drosha. Mol. Cell, 78(3): 423-439.

7. Zheng L, Chen Y, Li N, Zhang J, Liu N, Liu J, Dang W, Deng B, Li Y, Gao X, Tan C, Yang Z, Xu S, Wang M, Yang H, Sun L, Cui Y, Wei X, Gao P*, Wang HW*, Peng H*. (2020) Robust ultraclean atomically thin membranes for atomic-resolution electron microscopy. Nat. Commun., 11(1):541.

8. Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J*, Wang HW*. (2019) Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nat. Commun., 10(1):2386.

9. Liu N, Zhang J, Chen Y*, Liu C, Zhang X, Xu K, Wen J, Luo Z, Chen S, Gao P, Jia K, Liu Z, Peng H*, Wang HW*. (2019) Bioactive Functionalized Monolayer Graphene for High-Resolution Cryo-Electron Microscopy. J. Am. Chem. Soc., 141(9):4016-4025.

10. Wang J, Wang J, Hu M, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang HW*, Zhou JM*, Chai J*. (2019) Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364(6435). pii: eaav5868.

11. Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW*, Zhou JM*, Chai J*. (2019) Reconstitution and structure of a plant NLR resistosome conferring immunity. Science, 364(6435). pii: eaav5870.

12. Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang Q, Wang HW*. (2018) Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell, 173(5):1191-1203.

13. Fan X, Zhao L, Liu C, Zhang JC, Fan K, Yan X, Peng HL, Lei JL*, Wang HW*. (2017) Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM. Structure, 25(10):1623-1630.

14. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Liu Y, Zhao W, Zhou K, Liu QS, Dai J, Yang X, Dong MQ, Huang N, Wang HW*. (2016) CryoEM structure of yeast cytoplasmic exosome complex. Cell Research, 26(7):822-837.

15. Liu JJ, Bratkowski MA, Liu X, Niu CY, Ke A*, Wang HW*. (2014) Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM. Nat. Struct. Mol. Biol., 21(1):95-102.

●  联系方式：

电话：+86-10-62772390 (office)

+86-10-62798651 (lab)

E-mail: hongweiwang@tsinghua.edu.cn

Lab website: http://cryoem.life.tsinghua.edu.cn/